rabbit anti his6 igg conjugated to hrp  (Bethyl)

Journal: International Journal of Molecular Sciences

Article Title: Single-Domain Antibodies Represent Novel Alternatives to Monoclonal Antibodies as Targeting Agents against the Human Papillomavirus 16 E6 Protein

doi: 10.3390/ijms20092088

Figure Lengend Snippet: The HPV16 genome and E6 oncoprotein. ( A ) The circular, double-stranded DNA genome (~7.9 kbp) consists of a long control region (LCR) which regulates viral transcription, as well as seven early ( E1 , E2 , E4 , E5 , E6 , E7 , and E8 ) and two late ( L1 and L2 ) genes. The proteins encoded by the early genes promote viral persistence, replication, and release, whereas those encoded by the late genes create the viral capsid [ , , ]. The HPV16 genome (GenBank Accession #: K02718.1) was visualized using the Pathogen–Host Analysis Tool . ( B ) The E6 oncoprotein (~18 kDa) contains two zinc-binding domains. It interacts with host proteins after first complexing with the hijacked ubiquitin ligase E6AP or via its C-terminal PDZ-binding motif, driving cancerous changes in infected cells [ , ]. ( C ) Schematic representation of the recombinant HPV16 E6 proteins used in this study. Their amino acid differences from the reference sequence (GenBank Accession #: K02718.1) as well as their respective polyhistidine (His6) tags and maltose binding protein (MBP) fusion partners are indicated.

Article Snippet: The wells were then washed with PBS-T and incubated with a rabbit anti-His6 + HRP antibody (Cedarlane Laboratories; Cat. #: A-190-114P) diluted 1:20,000 in PBS (100 μL/well) for 1 h at room temperature.

Techniques: Control, Binding Assay, Ubiquitin Proteomics, Infection, Recombinant, Sequencing